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104 transcriptome library preparation  (Novogene)

 
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    Novogene 104 transcriptome library preparation
    104 Transcriptome Library Preparation, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptomic+libraries/104+library+preparation+transcriptome/10__1016_slash_j__hpj__2025__09__016-57-0-10
    Average 86 stars, based on 1 article reviews
    104 transcriptome library preparation - by Bioz Stars, 2026-10
    86/100 stars

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    RNA Sequencing:

    Article Title: Bornyl Diphosphate Synthase From Cinnamomum burmanni and Its Application for (+)-Borneol Biosynthesis in Yeast.
    Article Snippet: An aliquot containing 1 μg total RNA was used to synthesize the first-strand cDNA with TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotechnology, China) according to the manufacturer’s guidelines. .. Transcriptomic libraries of the C. burmannii leaves were shipped to the Novogene Company1 for library construction and RNA-seq. .. The Illumina-derived nucleotide sequences reported in this paper have been submitted to China National Center for Bioinformation2 under accession number CRA003558.

    Article Title: Bornyl Diphosphate Synthase From Cinnamomum burmanni and Its Application for (+)-Borneol Biosynthesis in Yeast
    Article Snippet: An aliquot containing 1 μg total RNA was used to synthesize the first-strand cDNA with TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen Biotechnology, China) according to the manufacturer’s guidelines. .. Transcriptomic libraries of the C. burmannii leaves were shipped to the Novogene Company for library construction and RNA-seq. .. The Illumina-derived nucleotide sequences reported in this paper have been submitted to China National Center for Bioinformation under accession number CRA003558 .



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    Changes in intestinal short chain fatty acids and spleen <t>transcriptome</t> in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.
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    Image Search Results


    Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in intestinal short chain fatty acids and spleen transcriptome in mice treated with SYD. (A) The boxplot of acetic acid abundance among three groups; (B) The boxplot of isobutyric acid abundance among three groups; (C) Differences in PISA scores among the three groups; (D) Differences in body weight among three groups of mice; (E) and (F) The difference in thickness between the left and right ears of mice among the three groups; (G) The correlation between short chain fatty acids and phenotype indicators in mice, yellow represents positive correlation, blue represents negative correlation, and darker colors indicate higher correlation coefficients; (H) The volcano plot of transcriptome between Psoriasis and SYD Treatment groups; (I) The heatmap of inflammation related genes among significant differentially expressed genes; (J) The dot plot of GO enrichment analysis of upregulated genes; (K) The GSEA analysis of the G protein-coupled receptor signaling pathway; (L) The analysis of CIBERSORT, which displayed the relative abundance of different cell subpopulations in three groups; (M) The box plot shows significant differences in the abundance of plasma, Treg, and Tfh cell subpopulations among the three groups.

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques: Clinical Proteomics

    Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Journal: Frontiers in Microbiology

    Article Title: Sanyin decoction alleviates psoriasis by reshaping gut microbiota and modulating the gut–spleen–skin axis

    doi: 10.3389/fmicb.2026.1799928

    Figure Lengend Snippet: Changes in transcriptome of mouse skin after SYD treatment. (A) The volcano of differentially expressed genes; (B) The GO bioprocess (BP) analysis of significant upregulated genes; (C) The KEGG analysis of significant upregulated genes; (D) The GO molecular function (MF) analysis of significant upregulated genes; (E)-(H) The GSEA enrichment of significant regulated genes, NES < 0, represents downregulated signaling pathway, adjusted p value < 0.05, represents statistically significant; (I) The GSEA enrichment of upregulated signaling pathway; (J) The result of skin immune infiltration between Psoriasis group and Treatment group using CIBERSORT; (K) Boxplot of significant differences in immune cell subpopulations (Macrophage_M2, Neutrophils, Dendritic resting_cells).

    Article Snippet: High-quality RNA was subsequently used for transcriptome library construction using the MGIEasy Fast RNA Reagent (MGI, 940-002921-00), mRNA was enriched using oligo (dT) magnetic beads, followed by fragmentation with fragmentation buffer at a controlled temperature.

    Techniques:

    a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: A DNA-based nanodevice senses purinergic signaling and drives an immune switch for resolving inflammation

    doi: 10.1038/s41467-026-68879-2

    Figure Lengend Snippet: a Schematic illustration of the RNA-seq and metabolomics analyses. b Gene cluster trend in different groups ( n = 3 mice). c GO enrichment analysis of genes in cluster 1. d GO enrichment analysis of genes in cluster 3. e GO enrichment analysis of genes in cluster 4. GO enrichment analysis was performed using a one-sided hypergeo metric test. Terms with a p < 0.05 were considered significantly enriched. f Heatmap o f the relative abundance of proinflammatory cytokines and purine metabolic genes in the three groups ( n = 3 mice). g PCA score plot of different groups subjected to metabolic analysis. h Pathway enrichment analysis of the DEMs enriched in ALI mice treated with ND@Monos (vs. the ALI group). Significance of enrichment was determined by a hypergeometric test with an FDR-corrected p -value ( q -value) threshold of 0.05. i , j Western blot analysis and quantification of IL-1β, P-creb and P-P65 levels ( n = 5 mice). Data are presented as mean ± SD. NC group indicates normal mice, and PBS indicates mice treated with vehicle. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.

    Article Snippet: The RNA sequencing transcriptome library was generated with a TruSeq TM RNA sample preparation kit from Illumina (San Diego, CA). cDNA was synthesized via a SuperScript double-stranded cDNA synthesis kit (Invitrogen) with random hexamer primers (Illumina).

    Techniques: RNA Sequencing, Western Blot, Comparison